AMBERCHROM™ XT20 is an excellent choice for the separation of synthetic oligonucleotides, or “oligos.” A single step purification of a 20-mer synthetic, phosphodiester oligonucleotides was developed on a 10 (ID) x 250 mm AMBERCHROM™ XT20 column. The crude DMT-on oligonucleotides which was not fully deprotected, was loaded onto the column, detritylated and eluted. One of the advantages of AMBERCHROM XT20 is the ability to detrytilate on-column with the use of 0.5% trifluoroacetic acid (TFA).
To address the dual needs for larger scale production and lower cost-of-goods produced for new therapeutic peptides, Dow has developed a full range of media for Reversed-Phased Chromatography (RPC) under the AMBERCHROM™ brand name. AMBERCHROM™ chromatographic media have distinguishing surface chemistries which offer distinctive selectivity for biomolecules. The effectiveness of these products has been demonstrated in commercial-scale production processes for therapeutic peptides for over a decade.
Due to its high resolving power, RPC is frequently used to purify peptides from either synthetic reaction mixtures or biological sources. Depending on the process requirements for throughput, capacity, and resolving power, an AMBERCHROM™ resin product can be used to help developers optimize their overall process.
A chemically robust chromatography resin can not only improve the cost of existing products, but can also enable the production of new products that might not otherwise be feasible to manufacture economically, as illustrated in the influenza peptide example.
Related Products
| Product | Description | Surface Area (m2/g) | Pore Size (Å) | Mean Diameter (microns) |
| AMBERCHROM™ CG300C | Chromatographic grade resin for use in many pharmaceutical applications in the front end capture, purification, and desalting modes of operation. | 700 | 300 | 120 |
| AMBERCHROM CG300M | Chromatographic grade resin designed for capture, purification, and polishing steps of downstream processing protocols of growth factors and other polypeptides, like insulin. | 700 | 300 | 75 |
| AMBERCHROM CG71M | For high value chromatographic applications, offering a high surface area and unique pore size and pore volume distribution. | 500 | 250 | 75 |
| AMBERCHROM CG71S | For high value chromatographic applications, offering a high surface area and unique pore size and pore volume distribution. | 500 | 250 | 30 |
| AMBERCHROM XT20 | Chromatographic grade resin for use in reversed phase chromatography for pharmaceutical and biopharmaceutical applications. | — | 300 | — |
Home Privacy
Terms of Use
Accessibility Statement
Sitemap
Media Center
®™* Trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow